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Molecular dimensions and condition: Much larger or irregularly formed molecules demand a broader pore measurement in the stationary section.
To reduce these troubles we place a guard column ahead of the analytical column. A Guard column typically consists of the same particulate packing subs
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If the outcome are to become reproducible, then the disorders of your separation need to even be reproducible. As a result HPLC gear need to be of high excellent; it can be hence pricey.
Detector – responds into the divided analytes emerging in the HPLC column and creates a sign output
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